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ELK Biotechnology
SKU:ELK1544
Human bCTx(Beta-Crosslaps) ELISA Kit
Human bCTx(Beta-Crosslaps) ELISA Kit
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$558.00 USD
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Alternative Names: Beta CTx; b-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction
Assay Type: Competitive Inhibition
Sensitivity: 36.16 pg/mL
standard: 8000 pg/mL
Detection range: 125-8000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2.5h
Research Area: Bone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human bCTx protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human bCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human bCTx in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 36.16 pg/mL
standard: 8000 pg/mL
Detection range: 125-8000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2.5h
Research Area: Bone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human bCTx protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human bCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human bCTx in the samples is then determined by comparing the OD of the samples to the standard curve.
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