Rat VIP(Vasoactive Intestinal Peptide) ELISA Kit

Alternative Names: PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27
Assay Type: Sandwich
Sensitivity: 2.7 pg/mL
standard: 500 pg/mL
Detection range: 7.82-500 pg/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Endocrinology;Gastroenterology;Hormone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat VIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VIP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P01283