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ELK Biotechnology
SKU:ELK1436
Human PAPPA(Pregnancy Associated Plasma Protein A) ELISA Kit
Human PAPPA(Pregnancy Associated Plasma Protein A) ELISA Kit
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Alternative Names: PAPP-A; PAPA; ASBABP2; DIPLA1; IGFBP-4ase; PAPPA1; Pappalysin 1; Insulin-Like Growth Factor Binding Protein-4 Protease; Differentially Placenta 1 Expressed Protein
Assay Type: Sandwich
Sensitivity: 0.12 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Reproductive science;Genetic science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAPPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAPPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAPPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAPPA in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q13219
Assay Type: Sandwich
Sensitivity: 0.12 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Reproductive science;Genetic science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAPPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAPPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAPPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAPPA in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q13219
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