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ELK Biotechnology
SKU:ELK1421
Human Flt3(FMS Like Tyrosine Kinase 3) ELISA Kit
Human Flt3(FMS Like Tyrosine Kinase 3) ELISA Kit
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Alternative Names: CD135; STK1; FLK2; Fms-Related Tyrosine Kinase 3; Stem Cell Tyrosine Kinase 1; FL Cytokine Receptor; Fetal Liver Kinase-2
Assay Type: Sandwich
Sensitivity: 0.115 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: CD & Adhesion molecule;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Flt3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Flt3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Flt3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Flt3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P36888
Assay Type: Sandwich
Sensitivity: 0.115 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: CD & Adhesion molecule;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Flt3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Flt3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Flt3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Flt3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P36888
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