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ELK Biotechnology
SKU:ELK1295
OVA(Ovalbumin) ELISA Kit
OVA(Ovalbumin) ELISA Kit
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$476.00 USD
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Alternative Names: Ovalbumin
Assay Type: Competitive Inhibition
Sensitivity: 46.9 pg/mL
standard: 10000 pg/mL
Detection range: 156.25-10000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2.5h
Research Area: Infection immunity;Immune molecule;Hematology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with OVA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to OVA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of OVA in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 46.9 pg/mL
standard: 10000 pg/mL
Detection range: 156.25-10000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2.5h
Research Area: Infection immunity;Immune molecule;Hematology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with OVA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to OVA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of OVA in the samples is then determined by comparing the OD of the samples to the standard curve.
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