1
/
of
1
ELK Biotechnology
SKU:ELK1249
Human IgG3(Immunoglobulin G3) ELISA Kit
Human IgG3(Immunoglobulin G3) ELISA Kit
Regular price
$476.00 USD
Regular price
Sale price
$476.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: IGHG3; HDC; Heavy chain disease protein; Ig Gamma-3 Chain C Region; Immunoglobulin Heavy Constant Gamma 3; G3m Marker; Immunoglobulin Gm3
Assay Type: Sandwich
Sensitivity: 1.49 µg/mL
standard: 200 µg/mL
Detection range: 3.13-200 µg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Infection immunity;Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IgG3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IgG3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IgG3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IgG3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P01860
Assay Type: Sandwich
Sensitivity: 1.49 µg/mL
standard: 200 µg/mL
Detection range: 3.13-200 µg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Infection immunity;Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IgG3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IgG3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IgG3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IgG3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P01860
Share
