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ELK Biotechnology
SKU:ELK1204
T4(Thyroxine) ELISA Kit
T4(Thyroxine) ELISA Kit
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$476.00 USD
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Alternative Names: 3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine
Assay Type: Competitive Inhibition
Sensitivity: 1.48 ng/mL
standard: 300 ng/mL
Detection range: 4.69-300 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Endocrinology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with T4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 1.48 ng/mL
standard: 300 ng/mL
Detection range: 4.69-300 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2.5h
Research Area: Endocrinology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with T4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T4 in the samples is then determined by comparing the OD of the samples to the standard curve.
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