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ELK Biotechnology
SKU:ELK11212
Pig HDL(High Density Lipoprotein) ELISA Kit
Pig HDL(High Density Lipoprotein) ELISA Kit
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$558.00 USD
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Alternative Names: High Density Lipoprotein
Assay Type: Sandwich
Sensitivity: 2.72 µg/mL
standard: 500 µg/mL
Detection range: 7.82-500 µg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig HDL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig HDL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig HDL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig HDL in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Sandwich
Sensitivity: 2.72 µg/mL
standard: 500 µg/mL
Detection range: 7.82-500 µg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;Nutrition metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig HDL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig HDL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig HDL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig HDL in the samples is then determined by comparing the OD of the samples to the standard curve.
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