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ELK Biotechnology
SKU:ELK11169
Rat AFM(Afamin) ELISA Kit
Rat AFM(Afamin) ELISA Kit
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$558.00 USD
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Alternative Names: ALB2; ALBA; ALF; A-ALB; Alpha-Albumin
Assay Type: Competitive Inhibition
Sensitivity: 0.89 µg/mL
standard: 200 µg/mL
Detection range: 3.13-200 µg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 2.5h
Research Area: Tumor immunity;Infection immunity;Hepatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AFM protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AFM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AFM in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P36953
Assay Type: Competitive Inhibition
Sensitivity: 0.89 µg/mL
standard: 200 µg/mL
Detection range: 3.13-200 µg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 2.5h
Research Area: Tumor immunity;Infection immunity;Hepatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AFM protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AFM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AFM in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P36953
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