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ELK Biotechnology
SKU:ELK11161
Mouse HRG(Histidine Rich Glycoprotein) ELISA Kit
Mouse HRG(Histidine Rich Glycoprotein) ELISA Kit
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Alternative Names: HPRG; HRGP; Histidine-Proline Rich Glycoprotein; Thrombophilia Due To Elevated HRG
Assay Type: Sandwich
Sensitivity: 0.129 µg/mLl
standard: 20 µg/mL
Detection range: 0.312-20 µg/mL
Sample type: Plasma.
Assay length: 3.5h
Research Area: Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HRG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HRG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HRG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HRG in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9ESB3
Assay Type: Sandwich
Sensitivity: 0.129 µg/mLl
standard: 20 µg/mL
Detection range: 0.312-20 µg/mL
Sample type: Plasma.
Assay length: 3.5h
Research Area: Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HRG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HRG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HRG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HRG in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9ESB3
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