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ELK Biotechnology
SKU:ELK11151
Rat HPX(Hemopexin) ELISA Kit
Rat HPX(Hemopexin) ELISA Kit
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Alternative Names: Haemopexin; Beta-1B-glycoprotein
Assay Type: Sandwich
Sensitivity: 0.62 µg/mL
standard: 100 µg/mL
Detection range: 1.56-100 µg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;Infection immunity;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HPX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HPX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HPX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HPX in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P20059
Assay Type: Sandwich
Sensitivity: 0.62 µg/mL
standard: 100 µg/mL
Detection range: 1.56-100 µg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;Infection immunity;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat HPX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HPX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat HPX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HPX in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P20059
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