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ELK Biotechnology
SKU:ELK11103
Human CFB(Complement Factor B) ELISA Kit
Human CFB(Complement Factor B) ELISA Kit
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Alternative Names: CF-B; BF; BFD; CFAB; GBG; H2-Bf; PBF2; B-Factor; Properdin; Properdin factor B; C3/C5 convertase; Glycine-rich beta glycoprotein
Assay Type: Sandwich
Sensitivity: 6.7 ng/mL
standard: 1000 ng/mL
Detection range: 15.63-1000 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Infection immunity;Immune molecule;Hematology;Autoimmunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CFB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CFB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CFB in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P00751
Assay Type: Sandwich
Sensitivity: 6.7 ng/mL
standard: 1000 ng/mL
Detection range: 15.63-1000 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Infection immunity;Immune molecule;Hematology;Autoimmunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CFB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CFB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CFB in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P00751
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