Human KLK10(Kallikrein 10) ELISA Kit

Alternative Names: NES1; PRSSL1; Kallikrein-Related Peptidase 10; Normal epithelial cell-specific 1; Protease serine-like 1
Assay Type: Competitive Inhibition
Sensitivity: 48.8 pg/mL
standard: 10000 pg/mL
Detection range: 156.25-10000 pg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 2.5h
Research Area: Enzyme & Kinase;Tumor immunity;Reproductive science;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK10 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK10 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O43240