Rabbit APOE(Apolipoprotein E) ELISA Kit

Alternative Names: Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset
Assay Type: Competitive Inhibition
Sensitivity: 0.25 µg/mL
standard: 50 µg/mL
Detection range: 0.78-50 µg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 2.5h
Research Area: Metabolic pathway;Cardiovascular biology;Hepatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rabbit APOE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit APOE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit APOE in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P18287