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ELK Biotechnology
SKU:ELK10874
Pig CLU(Clusterin) ELISA Kit
Pig CLU(Clusterin) ELISA Kit
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$688.00 USD
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Alternative Names: CLU; TRPM2; KUB1; CLI; APOJ; SGP2; AAG4; APO-J; SP-40; Apolipoprotein J; Complement Lysis Inhibitor; 40,Sulfated Glycoprotein 2; Testosterone-Repressed Prostate Message 2
Assay Type: Sandwich
Sensitivity: 0.45 µg/mL
standard: 100 µg/mL
Detection range: 1.57-100 µg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Apoptosis;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig CLU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig CLU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig CLU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig CLU in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q29549
Assay Type: Sandwich
Sensitivity: 0.45 µg/mL
standard: 100 µg/mL
Detection range: 1.57-100 µg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Apoptosis;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig CLU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig CLU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig CLU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig CLU in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q29549
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