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ELK Biotechnology
SKU:ELK10771
Chicken FASN(Fatty Acid Synthase) ELISA Kit
Chicken FASN(Fatty Acid Synthase) ELISA Kit
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Alternative Names: FAS; OA519; SDR27X1; Short Chain Dehydrogenase/Reductase Family 27X,Member 1; S-acetyltransferase; 3-oxoacyl synthase; Oleoyl hydrolase; Enoyl-acyl-carrier-protein reductase
Assay Type: Sandwich
Sensitivity: 0.128 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken FASN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken FASN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken FASN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken FASN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P12276
Assay Type: Sandwich
Sensitivity: 0.128 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Enzyme & Kinase;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken FASN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken FASN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken FASN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken FASN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P12276
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