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ELK Biotechnology
SKU:ELK10755
Rat AGC(Aggrecan) ELISA Kit
Rat AGC(Aggrecan) ELISA Kit
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Alternative Names: ACAN; AGC1; AGCAN; CSPG1; CSPCP; CSPGCP; MSK16; SEDK; Cartilage-specific proteoglycan core protein; Large Aggregating Proteoglycan; Chondroitin Sulfate Proteoglycan 1
Assay Type: Sandwich
Sensitivity: 0.152 µg/mL
standard: 20 µg/mL
Detection range: 0.32-20 µg/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Metabolic pathway;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AGC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AGC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AGC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AGC in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P07897
Assay Type: Sandwich
Sensitivity: 0.152 µg/mL
standard: 20 µg/mL
Detection range: 0.32-20 µg/mL
Sample type: Serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Metabolic pathway;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AGC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AGC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AGC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AGC in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P07897
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