1
/
of
1
ELK Biotechnology
SKU:ELK10744
Rat APLNR(Apelin Receptor) ELISA Kit
Rat APLNR(Apelin Receptor) ELISA Kit
Regular price
$558.00 USD
Regular price
Sale price
$558.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: AGTRL1; HG11; APJR; APJ; APJ Receptor; Angiotensin II Receptor-Like 1; Angiotensin receptor-like 1
Assay Type: Sandwich
Sensitivity: 9.8 pg/mL
standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Endocrinology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat APLNR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APLNR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat APLNR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat APLNR in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9JHG3
Assay Type: Sandwich
Sensitivity: 9.8 pg/mL
standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Endocrinology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat APLNR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APLNR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat APLNR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat APLNR in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9JHG3
Share
