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ELK Biotechnology
SKU:ELK10705
Cattle TSP-1(Thrombospondin-1) ELISA Kit
Cattle TSP-1(Thrombospondin-1) ELISA Kit
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Alternative Names: TSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1
Assay Type: Sandwich
Sensitivity: 0.43 ng/mL
standard: 100 ng/mL
Detection range: 1.56-100 ng/mL
Sample type: Serum and plasma
Assay length: 3.5h
Research Area: Infection immunity;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q28178
Assay Type: Sandwich
Sensitivity: 0.43 ng/mL
standard: 100 ng/mL
Detection range: 1.56-100 ng/mL
Sample type: Serum and plasma
Assay length: 3.5h
Research Area: Infection immunity;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q28178
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