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ELK Biotechnology
SKU:ELK10641
Mouse PⅠNP(Procollagen Ⅰ N-Terminal Propeptide) ELISA Kit
Mouse PⅠNP(Procollagen Ⅰ N-Terminal Propeptide) ELISA Kit
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$558.00 USD
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Alternative Names: P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide; PINP; Procollagen I N-Terminal Propeptide
Assay Type: Sandwich
Sensitivity: 0.5662 ng/mL
standard: 100 ng/mL
Detection range: 1.56-100 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Hepatology;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PⅠNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PⅠNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PⅠNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PⅠNP in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Sandwich
Sensitivity: 0.5662 ng/mL
standard: 100 ng/mL
Detection range: 1.56-100 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Hepatology;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PⅠNP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PⅠNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PⅠNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PⅠNP in the samples is then determined by comparing the OD of the samples to the standard curve.
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