1
/
of
1
ELK Biotechnology
SKU:ELK10607
Chicken OC(Osteocalcin) ELISA Kit
Chicken OC(Osteocalcin) ELISA Kit
Regular price
$688.00 USD
Regular price
Sale price
$688.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein
Assay Type: Sandwich
Sensitivity: 0.064 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken OC in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P02822
Assay Type: Sandwich
Sensitivity: 0.064 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Hormone metabolism;Bone metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken OC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken OC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken OC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken OC in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P02822
Share
