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ELK Biotechnology
SKU:ELK10587
Mouse FABP4(Fatty Acid Binding Protein 4, Adipocyte) ELISA Kit
Mouse FABP4(Fatty Acid Binding Protein 4, Adipocyte) ELISA Kit
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Alternative Names: A-FABP; AFABP; AP2; Adipocyte Protein 2; Adipocyte-type fatty acid-binding protein
Assay Type: Sandwich
Sensitivity: 0.18 ng/mL
standard: 50 ng/mL
Detection range: 0.78-50 ng/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FABP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FABP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FABP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FABP4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P04117
Assay Type: Sandwich
Sensitivity: 0.18 ng/mL
standard: 50 ng/mL
Detection range: 0.78-50 ng/mL
Sample type: serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;Endocrinology;Cardiovascular biology;Hepatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FABP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FABP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FABP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FABP4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P04117
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