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ELK Biotechnology
SKU:ELK10586
Mouse DBP(Vitamin D Binding Protein) ELISA Kit
Mouse DBP(Vitamin D Binding Protein) ELISA Kit
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Alternative Names: GC; VDBG; VDBP; Group-Specific Component; Gc-globulin
Assay Type: Sandwich
Sensitivity: 0.68 µg/mL
standard: 100 µg/mL
Detection range: 1.57-100 µg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DBP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DBP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P21614
Assay Type: Sandwich
Sensitivity: 0.68 µg/mL
standard: 100 µg/mL
Detection range: 1.57-100 µg/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Metabolic pathway;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DBP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DBP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P21614
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