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ELK Biotechnology
SKU:ELK10577
Mouse APAF1(Apoptotic Peptidase Activating Factor 1) ELISA Kit
Mouse APAF1(Apoptotic Peptidase Activating Factor 1) ELISA Kit
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Alternative Names: CED4; Apoptotic Protease Activating Factor
Assay Type: Sandwich
Sensitivity: 0.054 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Apoptosis;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse APAF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APAF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse APAF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APAF1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O88879
Assay Type: Sandwich
Sensitivity: 0.054 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Tissue homogenates, cell lysates and other biological fluids.
Assay length: 3.5h
Research Area: Apoptosis;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse APAF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APAF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse APAF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APAF1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O88879
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