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ELK Biotechnology
SKU:ELK10575
Mouse HCAM(Homing Associated Cell Adhesion Molecule) ELISA Kit
Mouse HCAM(Homing Associated Cell Adhesion Molecule) ELISA Kit
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$558.00 USD
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Alternative Names: CDW44; CD44; CSPG8; ECMR-III; HCELL; IN; LHR; MC56; MDU2; MDU3; MIC4; MUTCH-I; Pgp1; Hematopoietic Cell E- And L-Selectin Ligand; Chondroitin Sulfate Proteoglycan 8
Assay Type: Sandwich
Sensitivity: 0.061 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HCAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HCAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HCAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HCAM in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Sandwich
Sensitivity: 0.061 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HCAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HCAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HCAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HCAM in the samples is then determined by comparing the OD of the samples to the standard curve.
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