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ELK Biotechnology

SKU:ELK10556

Human C1qC(Complement Component 1, Q Subcomponent C) ELISA Kit

Human C1qC(Complement Component 1, Q Subcomponent C) ELISA Kit

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Alternative Names: C1QG; Complement Component 1 q subcomponent,Gamma Polypeptide
Assay Type: Sandwich
Sensitivity: 1.29 ng/mL
standard: 200 ng/mL
Detection range: 3.13-200 ng/mL
Sample type: Serum, plasma and other biological fluids.
Assay length: 3.5h
Research Area: Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C1qC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C1qC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C1qC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C1qC in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P02747
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