1
/
of
1
ELK Biotechnology
SKU:ELK10479
Mouse F8(Coagulation Factor VIII) ELISA Kit
Mouse F8(Coagulation Factor VIII) ELISA Kit
Regular price
$558.00 USD
Regular price
Sale price
$558.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: FVIII; AHF; HEMA; F8B; F8C; Antihemophilic Globulin; Hemophilia A; Anti-Hemophilic Factor A; FVIII Procoagulant Component; F8 Protein
Assay Type: Sandwich
Sensitivity: 0.081 IU/mL
standard: 20 IU/mL
Detection range: 0.32-20 IU/mL
Sample type: plasma
Assay length: 3.5h
Research Area: Metabolic pathway;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse F8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse F8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse F8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse F8 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q06194
Assay Type: Sandwich
Sensitivity: 0.081 IU/mL
standard: 20 IU/mL
Detection range: 0.32-20 IU/mL
Sample type: plasma
Assay length: 3.5h
Research Area: Metabolic pathway;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse F8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse F8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse F8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse F8 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q06194
Share
