1
/
of
1
ELK Biotechnology
SKU:ELK10407
Rat SELP(Selectin, Platelet) ELISA Kit
Rat SELP(Selectin, Platelet) ELISA Kit
Regular price
$558.00 USD
Regular price
Sale price
$558.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: CD62P; P-Selectin; PSEL; GMP140; LECAM3; GRMP; GMRP; PADGEM; Leukocyte-endothelial cell adhesion molecule 3; Platelet activation dependent granule-external membrane
Assay Type: Sandwich
Sensitivity: 2.853 ng/mL
standard: 400 ng/mL
Detection range: 6.25-400 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SELP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SELP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SELP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SELP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P98106
Assay Type: Sandwich
Sensitivity: 2.853 ng/mL
standard: 400 ng/mL
Detection range: 6.25-400 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat SELP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat SELP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat SELP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat SELP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P98106
Share
