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ELK Biotechnology
SKU:ELK10400
Mouse ELA2(Elastase 2, Neutrophil) ELISA Kit
Mouse ELA2(Elastase 2, Neutrophil) ELISA Kit
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Alternative Names: ELANE; HLE; HNE; NE; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Human Leukocyte Elastase; PMN Elastase
Assay Type: Sandwich
Sensitivity: 0.57 ng/mL
standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ELA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ELA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ELA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ELA2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q3UP87
Assay Type: Sandwich
Sensitivity: 0.57 ng/mL
standard: 100 ng/mL
Detection range: 1.57-100 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ELA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ELA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ELA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ELA2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q3UP87
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