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ELK Biotechnology
SKU:ELK10355
Human PDIA3(Protein Disulfide Isomerase A3) ELISA Kit
Human PDIA3(Protein Disulfide Isomerase A3) ELISA Kit
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Alternative Names: P58; ERp61; ERp57; ERp60; GRP57; PI-PLC; GRP58; Glucose Regulated Protein,58kDa; Protein Disulfide Isomerase-Associated 3; Endoplasmic reticulum resident protein 57
Assay Type: Sandwich
Sensitivity: 0.46 ng/mL
standard: 100 ng/mL
Detection range: 1.56-100 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDIA3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDIA3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDIA3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDIA3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P30101
Assay Type: Sandwich
Sensitivity: 0.46 ng/mL
standard: 100 ng/mL
Detection range: 1.56-100 ng/mL
Sample type: tissue homogenates, cell lysates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDIA3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDIA3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDIA3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDIA3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P30101
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