1
/
of
1
ELK Biotechnology
SKU:ELK10339
Human HCII(Heparin Cofactor II) ELISA Kit
Human HCII(Heparin Cofactor II) ELISA Kit
Regular price
$558.00 USD
Regular price
Sale price
$558.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: SERPIND1; HC2; HCF2; HC II; HLS2; LS2; Serpin D1; Serpin Peptidase Inhibitor Clade D Member 1; Protease inhibitor leuserpin-2
Assay Type: Sandwich
Sensitivity: 0.13 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Plasma
Assay length: 3.5h
Research Area: Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HCII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HCII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HCII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HCII in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P05546
Assay Type: Sandwich
Sensitivity: 0.13 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Plasma
Assay length: 3.5h
Research Area: Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HCII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HCII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HCII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HCII in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P05546
Share
