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ELK Biotechnology
SKU:ELK10301
Mouse DPP4(Dipeptidyl Peptidase IV) ELISA Kit
Mouse DPP4(Dipeptidyl Peptidase IV) ELISA Kit
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Alternative Names: CD26; ADA; DPPIV; DPP-IV; ADABP; ADCP2; TP103; Adenosine Deaminase Complexing Protein 2; T-cell activation antigen CD26
Assay Type: Sandwich
Sensitivity: 0.21 ng/mL
standard: 40 ng/mL
Detection range: 0.63-40 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity;Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DPP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DPP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DPP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DPP4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P28843
Assay Type: Sandwich
Sensitivity: 0.21 ng/mL
standard: 40 ng/mL
Detection range: 0.63-40 ng/mL
Sample type: Serum, plasma, tissue homogenates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity;Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DPP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DPP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DPP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DPP4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P28843
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