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ELK Biotechnology
SKU:ELK10292
Human PAP(Plasmin/Antiplasmin Complex) ELISA Kit
Human PAP(Plasmin/Antiplasmin Complex) ELISA Kit
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$558.00 USD
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Alternative Names: Plasmin/Antiplasmin Complex
Assay Type: Sandwich
Sensitivity: 2.3 ng/mL
standard: 500 ng/mL
Detection range: 7.81-500 ng/mL
Sample type: plasma
Assay length: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAP in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Sandwich
Sensitivity: 2.3 ng/mL
standard: 500 ng/mL
Detection range: 7.81-500 ng/mL
Sample type: plasma
Assay length: 3.5h
Research Area: Signal transduction;Enzyme & Kinase;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAP in the samples is then determined by comparing the OD of the samples to the standard curve.
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