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ELK Biotechnology
SKU:ELK10192
Human CLU(Clusterin) ELISA Kit
Human CLU(Clusterin) ELISA Kit
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$476.00 USD
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Alternative Names: CLU; TRPM2; KUB1; CLI; APOJ; SGP2; AAG4; APO-J; SP-40; Apolipoprotein J; Complement Lysis Inhibitor; 40,Sulfated Glycoprotein 2; Testosterone-Repressed Prostate Message 2
Assay Type: Sandwich
Sensitivity: 0.57 µg/mL
standard: 100 µg/mL
Detection range: 1.57-100 µg/mL
Sample type: serum, plasma, urine, saliva, tissue homogenates, cerebrospinal fluid, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Apoptosis;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CLU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CLU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CLU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CLU in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O75153
Assay Type: Sandwich
Sensitivity: 0.57 µg/mL
standard: 100 µg/mL
Detection range: 1.57-100 µg/mL
Sample type: serum, plasma, urine, saliva, tissue homogenates, cerebrospinal fluid, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Apoptosis;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CLU. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CLU. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CLU, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CLU in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O75153
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