Mouse KRT10(Keratin 10) ELISA Kit

Alternative Names: K10; CK10; KPP; Cytokeratin 10; Epidermolytic Hyperkeratosis; Keratosis Palmaris Et Plantaris; Keratin, type I cytoskeletal 10
Assay Type: Sandwich
Sensitivity: 0.12 µg/mL
standard: 20 µg/mL
Detection range: 0.32-20 µg/mL
Sample type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Tumor immunity;Infection immunity;Dermatology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse KRT10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KRT10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse KRT10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KRT10 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P02535