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ELK Biotechnology
SKU:ELK10104
Mouse vWF(Von Willebrand Factor) ELISA Kit
Mouse vWF(Von Willebrand Factor) ELISA Kit
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Alternative Names: F8VWF; VWD; von Willebrand antigen 2
Assay Type: Sandwich
Sensitivity: 0.049 µg/mL
standard: 10 µg/mL
Detection range: 0.16-10 µg/mL
Sample type: plasma
Assay length: 3.5h
Research Area: Signal transduction;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse vWF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse vWF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse vWF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse vWF in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q8CIZ8
Assay Type: Sandwich
Sensitivity: 0.049 µg/mL
standard: 10 µg/mL
Detection range: 0.16-10 µg/mL
Sample type: plasma
Assay length: 3.5h
Research Area: Signal transduction;Hematology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse vWF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse vWF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse vWF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse vWF in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q8CIZ8
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