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ELK Biotechnology
SKU:ELK10032
Human PGF2α(Prostaglandin F2 Alpha) ELISA Kit
Human PGF2α(Prostaglandin F2 Alpha) ELISA Kit
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$558.00 USD
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Alternative Names: 8-isoprostane; 8-iso-PGF2α
Assay Type: Competitive Inhibition
Sensitivity: 3.65 pg/mL
standard: 800 pg/mL
Detection range: 12.5-800 pg/mL
Sample type: Serum, plasma, saliva and other biological fluids.
Assay length: 2.5h
Research Area: Metabolic pathway;Cardiovascular biology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PGF2α protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 3.65 pg/mL
standard: 800 pg/mL
Detection range: 12.5-800 pg/mL
Sample type: Serum, plasma, saliva and other biological fluids.
Assay length: 2.5h
Research Area: Metabolic pathway;Cardiovascular biology;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PGF2α protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.
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