1
/
of
1
ELK Biotechnology
SKU:ELK10031
Human CST7(Cystatin-F) ELISA Kit
Human CST7(Cystatin-F) ELISA Kit
Regular price
$558.00 USD
Regular price
Sale price
$558.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: CST7;Cystatin-F;Cystatin-7;Cystatin-like metastasis-associated protein ;CMAP;Leukocystatin
Assay Type: Sandwich
Sensitivity: 0.156 ng/mL
standard: 40 ng/mL
Detection range: 0.625-40 ng/mL
Sample type: Serum, plasma,cell culture supernates
Assay length: 3.5h
Research Area: Cell Biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CST7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CST7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CST7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CST7 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O76096
Assay Type: Sandwich
Sensitivity: 0.156 ng/mL
standard: 40 ng/mL
Detection range: 0.625-40 ng/mL
Sample type: Serum, plasma,cell culture supernates
Assay length: 3.5h
Research Area: Cell Biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CST7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CST7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CST7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CST7 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O76096
Share
