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ELK Biotechnology
SKU:ELK0922
Human SMN1(Survival Of Motor Neuron 1, Centromeric) ELISA Kit
Human SMN1(Survival Of Motor Neuron 1, Centromeric) ELISA Kit
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Alternative Names: SMA; SMN; SMA1; SMA2; SMA3; SMA4; SMA@; SMNT; BCD541; GEMIN1; TDRD16A; T-BCD541
Assay Type: Sandwich
Sensitivity: 13.4 pg/mL
standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SMN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SMN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SMN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SMN1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q16637
Assay Type: Sandwich
Sensitivity: 13.4 pg/mL
standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SMN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SMN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SMN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SMN1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q16637
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