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ELK Biotechnology
SKU:ELK0891
Human C3b(Complement Fragment 3b) ELISA Kit
Human C3b(Complement Fragment 3b) ELISA Kit
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Alternative Names: AHUS5; ARMD9; ASPa; C3b; CPAMD1; HEL-S-62p; C3
Assay Type: Sandwich
Sensitivity: 0.12 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Infection immunity;Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C3b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C3b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C3b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C3b in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P01024
Assay Type: Sandwich
Sensitivity: 0.12 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;Infection immunity;Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C3b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C3b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C3b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C3b in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P01024
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