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ELK Biotechnology
SKU:ELK0734
Human M2BPGi(Mac-2 Binding Protein Glycosylation Isomer) ELISA Kit
Human M2BPGi(Mac-2 Binding Protein Glycosylation Isomer) ELISA Kit
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Alternative Names: M2BPGI
Assay Type: Sandwich
Sensitivity: 6.91 pg/mL
standard: 1000 pg/mL
Detection range: 15.63-1000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human M2BPGi. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human M2BPGi. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human M2BPGi, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human M2BPGi in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Sandwich
Sensitivity: 6.91 pg/mL
standard: 1000 pg/mL
Detection range: 15.63-1000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human M2BPGi. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human M2BPGi. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human M2BPGi, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human M2BPGi in the samples is then determined by comparing the OD of the samples to the standard curve.
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