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ELK Biotechnology
SKU:ELK072ES
EasyStep Human EGFR(Epidermal Growth Factor Receptor) ELISA Kit
EasyStep Human EGFR(Epidermal Growth Factor Receptor) ELISA Kit
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$558.00 USD
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Alternative Names: ErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Assay Type: Sandwich
Sensitivity: 25 pg/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma
Assay length: 1.5h
Research Area: CD & Adhesion molecule;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGFR, and the Human EGFR standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human EGFR. After TMB substrate solution is added, only those wells that contain Human EGFR and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGFR in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Sandwich
Sensitivity: 25 pg/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: Serum, plasma
Assay length: 1.5h
Research Area: CD & Adhesion molecule;Tumor immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGFR, and the Human EGFR standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human EGFR. After TMB substrate solution is added, only those wells that contain Human EGFR and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGFR in the samples is then determined by comparing the OD of the samples to the standard curve.
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