1
/
of
1
ELK Biotechnology
SKU:ELK066ES
EasyStep Human TRF(Transferrin) ELISA Kit
EasyStep Human TRF(Transferrin) ELISA Kit
Regular price
$558.00 USD
Regular price
Sale price
$558.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: TF; Siderophilin; Serotransferrin; Beta-1 metal-binding globulin
Assay Type: Sandwich
Sensitivity: 11.9 ng/mL
standard: 1000 ng/mL
Detection range: 15.63-1000 ng/mL
Sample type: Serum, plasma
Assay length: 1.5h
Research Area: Tumor immunity;Infection immunity;Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRF, and the Human TRF standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human TRF. After TMB substrate solution is added, only those wells that contain Human TRF and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRF in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Sandwich
Sensitivity: 11.9 ng/mL
standard: 1000 ng/mL
Detection range: 15.63-1000 ng/mL
Sample type: Serum, plasma
Assay length: 1.5h
Research Area: Tumor immunity;Infection immunity;Immune molecule;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRF, and the Human TRF standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human TRF. After TMB substrate solution is added, only those wells that contain Human TRF and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRF in the samples is then determined by comparing the OD of the samples to the standard curve.
Share
