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ELK Biotechnology
SKU:ELK0534
Rat NUP62(Nucleoporin 62kDa) ELISA Kit
Rat NUP62(Nucleoporin 62kDa) ELISA Kit
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Alternative Names: IBSN; p62; SNDI; Nuclear Pore Glycoprotein p62
Assay Type: Sandwich
Sensitivity: 0.14 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;Developmental science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NUP62. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NUP62. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NUP62, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NUP62 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P17955
Assay Type: Sandwich
Sensitivity: 0.14 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Signal transduction;Developmental science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NUP62. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NUP62. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NUP62, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NUP62 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P17955
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