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ELK Biotechnology
SKU:ELK0395
Human ARHGEF2(Rho Guanine Nucleotide Exchange Factor 2) ELISA Kit
Human ARHGEF2(Rho Guanine Nucleotide Exchange Factor 2) ELISA Kit
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Alternative Names: ARHG2; Guanine nucleotide exchange factor H1; Lfc; GEFH1; NEDMHM; LFP40; GEF-H1; P40; GEF
Assay Type: Sandwich
Sensitivity: 16.1 pg/mL
standard: 3000 pg/mL
Detection range: 46.88-3000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ARHGEF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ARHGEF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ARHGEF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ARHGEF2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Sandwich
Sensitivity: 16.1 pg/mL
standard: 3000 pg/mL
Detection range: 46.88-3000 pg/mL
Sample type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Signal transduction;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ARHGEF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ARHGEF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ARHGEF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ARHGEF2 in the samples is then determined by comparing the OD of the samples to the standard curve.
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