Mouse FUNDC1(FUN14 domain-containing protein 1) ELISA Kit

Alternative Names: FUNDC1; FUN14 domain-containing protein 1;
Assay Type: Sandwich
Sensitivity: 11.5 pg/mL
standard: 2000 pg/mL
Detection range: 31.25-2000 pg/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Cell Biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FUNDC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FUNDC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FUNDC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FUNDC1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q9DB70