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ELK Biotechnology
SKU:ELK0269
Human STOM(Stomatin) ELISA Kit
Human STOM(Stomatin) ELISA Kit
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Alternative Names: Erythrocyte band 7 integral membrane protein; Erythrocyte membrane protein band 7.2; Protein 7.2b
Assay Type: Sandwich
Sensitivity: 0.11 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human STOM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human STOM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human STOM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human STOM in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P27105
Assay Type: Sandwich
Sensitivity: 0.11 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length: 3.5h
Research Area: Metabolic pathway;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human STOM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human STOM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human STOM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human STOM in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P27105
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