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ELK Biotechnology
SKU:ELK022ES
EasyStep Rat E2(Estradiol) ELISA Kit
EasyStep Rat E2(Estradiol) ELISA Kit
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$558.00 USD
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Alternative Names: 17B-Estradiol; Oestradiol; Beta-Estradiol
Assay Type: Competitive Inhibition
Sensitivity: 25 pg/mL
standard: 1600 pg/mL
Detection range: 25-1600 pg/mL
Sample type: Serum
Assay length: 1.5h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat E2 antigen, and the Rat E2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat E2 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Type: Competitive Inhibition
Sensitivity: 25 pg/mL
standard: 1600 pg/mL
Detection range: 25-1600 pg/mL
Sample type: Serum
Assay length: 1.5h
Research Area: Endocrinology;Reproductive science;Hormone metabolism;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat E2 antigen, and the Rat E2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat E2 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
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