1
/
of
1
ELK Biotechnology
SKU:ELK0214
Rat GAPDH(Glyceraldehyde-3-Phosphate Dehydrogenase) ELISA Kit
Rat GAPDH(Glyceraldehyde-3-Phosphate Dehydrogenase) ELISA Kit
Regular price
$558.00 USD
Regular price
Sale price
$558.00 USD
Unit price
/
per
Shipping calculated at checkout.
Couldn't load pickup availability
Alternative Names: G3PD; GAPD; Peptidyl-cysteine S-nitrosylase GAPDH
Assay Type: Sandwich
Sensitivity: 0.067 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Cardiovascular biology;Hepatology;Nutrition metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GAPDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GAPDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GAPDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GAPDH in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P04797
Assay Type: Sandwich
Sensitivity: 0.067 ng/mL
standard: 10 ng/mL
Detection range: 0.16-10 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 3.5h
Research Area: Enzyme & Kinase;Metabolic pathway;Cardiovascular biology;Hepatology;Nutrition metabolism;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GAPDH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GAPDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GAPDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GAPDH in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P04797
Share
