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ELK Biotechnology
SKU:ELK0108
Rat CHRNA2(Neuronal Acetylcholine Receptor Subunit Alpha-2) ELISA Kit
Rat CHRNA2(Neuronal Acetylcholine Receptor Subunit Alpha-2) ELISA Kit
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Alternative Names: CHR-NA2; CHRN-A2; N-AChRA2; NAChRA2; N-AChR-A2; Neuronal Acetylcholine Receptor Alpha 2
Assay Type: Sandwich
Sensitivity: 0.11 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CHRNA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CHRNA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CHRNA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CHRNA2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P12389
Assay Type: Sandwich
Sensitivity: 0.11 ng/mL
standard: 20 ng/mL
Detection range: 0.32-20 ng/mL
Sample type: Tissue homogenates and other biological fluids.
Assay length: 3.5h
Research Area: Neuro science;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CHRNA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CHRNA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CHRNA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CHRNA2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P12389
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